Mistranslation
and
Resistance to Antibiotics
The year ended on a wonderful note. Deeply grateful to SMBE for awarding Shraddha Karve and myself a generous grant, leading to the SMBE regional meeting in India at Dehradun this year! Titled ‘Molecular mechanisms in evolution’, we truly enjoyed organizing it and hope the attendees felt the same way. The serene surroundings and great science came together
We now have the SERB start-up grant for work that will explore links between mistranslation and AMR- yay! Plus, we are very grateful to Axis bank for supporting our work on AMR: environmental surveillance as well as laboratory based experimental evolution.
Laasya Samhita and Shraddha Karve are trying to bring together the (too) small community of researchers in India who work at the interface of molecular biology and evolution at Ashoka university in December this year. Updated: NEMO (New Ideas in Molecular Evolution) was much fun, and succeeded in getting together a bunch of (largely) early career biologists straddling
Hear Laasya bust three common myths about science and scientists – https://youtu.be/UFEJ8pxqCg4 (23/8/2022)
Usually, the genetic code is decoded in sets of three nucleotides starting with the start codon. Occasionally, the decoding ribosomes slip and change the ‘reading frame’. For some viruses, changing reading frames is a way of life. Read on to find out more about a particular study that I have covered in this clipboard: https://www.ias.ac.in/describe/article/jbsc/047/0049
An old article of Laasya’s on the Handicap principle has been included in the special 75-year e-book of Resonance! Aimed at high school and college students. https://www.ias.ac.in/Publications/e-Books/Resonance-75-vol1
Welcome to MIRA Labs! While we understand how DNA based changes contribute to adaptation and evolution, the impact of non-genetic variation has remained controversial. We work with mistranslation, one of the biggest and most widespread contributors to non-genetic variation. In prior work, we found that specific protein changes may be unimportant, instead, stress responses triggered by mistranslated proteins could aid in adaptation. How does mistranslation contribute to survival under stress? Do all cells in population mistranslate to the same degree, what does this mean for adaptation? Can protein-only changes feed back into DNA changes and contribute to evolution? Most assessments of antibiotic resistance explore only genetic resistance. We found that basal resistance to the antibiotic ciprofloxacin requires some amount of mistranslation by the cell. How can we identify non-genetic drivers of resistance and quantify their contribution to the AMR problem? How do translation rate and accuracy impact resistance to antibiotics? Can we design drugs to target non-mutational tolerance? We use a combination of molecular biology and biochemistry techniques, experimental evolution, microscopy and collaborations as needed to address these questions!
Seed funds from TSB
Rockefeller foundation (via APSI: https://data.ccmb.res.in/apsi/about/)
Axis bank
SERB start-up grant (awarded September 2023, funds awaited)